Dr Raman Vaidyanathan
School of Chemical, Materials and Biological Engineering
Senior Lecturer in Chemical Engineering
Course Director MSc (Eng) Biological and Bioprocess Engineering
Course Director MSc (Eng) Biochemical Engineering with Industrial Management
+44 114 222 7526
Full contact details
School of Chemical, Materials and Biological Engineering
Room G60
Sir Robert Hadfield Building
Mappin Street
91直播
S1 3JD
- Profile
-
I joined the Department as a lecturer in 2007. I'm a chemical graduate with a Masters degree in Biotechnology (both from India) and completed my PhD in 2001 from the University of Strathclyde in Glasgow, where I investigated the application of near infrared spectroscopy to monitor industrially relevant bioprocesses involving filamentous microorganisms. This was work done in collaboration with Eli Lilly.
I have over five years of pre-doctoral industrial experience applying biotechnological solutions to environmental engineering problems.
Before joining 91直播 I was a research associate at the University of Manchester, where I investigated a) the application of secondary ion mass spectrometry with buckminsterfullerene (C60) cluster ions as primary ion sources to characterize biological surfaces, and b) laser desorption ionization mass spectrometry for metabolomic applications.
I also spent my post-doctoral years investigating the application of soft ionization mass spectrometry to characterize biological systems, at Aberystwyth in Wales.
- Research interests
-
Algae Biotechnology
Systems and synthetic biology
Bioprocess Engineering
Bioenergy
Bioinformatics and use of machine learning
Metabolomics and Proteomics
Mass spectrometric imaging
- Publications
-
Books
Journal articles
- . Chemical Engineering Journal, 458, 141433-141433.
- . Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 270.
- . Bioresource Technology.
- . Heliyon, 7(8), e07676-e07676.
- . iScience, 102743-102743.
- . Critical Reviews in Biotechnology.
- . Separation and Purification Technology, 255.
- . Biology, 9(8).
- . Applied Energy, 261.
- . Trends in Biotechnology.
- . Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 224.
- . Scientific Reports, 9.
- . Metabolites, 8(4).
- . Metabolites, 8(4).
- . Biotechnology for Biofuels, 11(1), 60-60.
- . Critical Reviews in Biotechnology.
- . Critical Reviews in Biotechnology, 38(5), 690-703.
- . Analyst, 142(11), 2038-2049.
- . Philosophical transactions of the Royal Society of London. Series A: Mathematical and physical sciences, 374.
- . Algal Research, 18, 213-224.
- . Current Biotechnology, 5(4), 305-313.
- . Science of The Total Environment, 541, 1282-1295.
- . Metabolomics, 11(6), 1743-1755.
- . Energy Conversion and Management, 106, 61-72.
- . Bioinformatics, 31(5), 753-760.
- . Anal Chim Acta, 776, 31-40.
- . J Proteome Res, 11(12), 5959-5971.
- . Analyst, 137(10), 2350-2356.
- A simple, reproducible and sensitive spectrophotometric method to estimate microalgal lipids. Analytica Chimica Acta, 724, 67-72.
- . Analytica Chimica Acta.
- . Analytica Chimica Acta.
- . Mol Biosyst, 7(1), 101-115.
- . Analyst, 134(11), 2352-2360.
- . APPLIED SURFACE SCIENCE, 255(4), 1305-1306.
- . Molecular Biotechnology, 40(2), 214-215.
- . Anal Chem, 80(6), 1942-1951.
- . Anal Chem, 79(6), 2199-2206.
- , 29-48.
- . Rapid Commun Mass Spectrom, 21(13), 2072-2078.
- . Rapid Commun Mass Spectrom, 21(13), 2157-2166.
- Influence of instrumental conditions on the electrospray ionization mass spectrometry of peptides/proteins in mixtures. American Biotechnology Laboratory, 24(2), 32-33.
- . Rapid Commun Mass Spectrom, 20(1), 21-30.
- . Rapid Commun Mass Spectrom, 20(8), 1192-1198.
- . METABOLOMICS, 1(3), 243-250.
- . METABOLOMICS, 1(1), 17-28.
- . Metabolome Analyses: Strategies for Systems Biology, 1-7.
- . Metabolome Analyses: Strategies for Systems Biology, 1-390.
- . Chemical Analysis, 169, 229-256.
- . Anal Chem, 76(17), 5024-5032.
- . Trends Biotechnol, 22(5), 245-252.
- . Genome Biol, 5(11), 354.
- . Anal Chem, 75(23), 6679-6686.
- . BIOTECHNOLOGY AND BIOENGINEERING, 82(6), 715-724.
- Metabolic profiling using direct infusion electrospray ionisation mass spectrometry for the characterisation of olive oils.. Analyst, 127(11), 1457-1462.
- Flow-injection electrospray ionisation mass spectrometry of crude cell extracts for high-throughput bacterial identification.. Journal of the American Society for Mass Spectrometry, 13, 118-128.
- Sample preparation in matrix鈥恆ssisted laser desorption/ionization mass spectrometry of whole bacterial cells and the detection of high mass (> 20 kDa) proteins.. Rapid Communications in Mass Spectrometry, 16(13), 1276-1286.
- . Biotechnol Bioeng, 74(5), 376-388.
- . ANALYTICAL CHEMISTRY, 73(17), 4134-4144.
- . APPLIED SPECTROSCOPY, 55(4), 444-453.
- . ANALYTICA CHIMICA ACTA, 428(1), 41-59.
- . Enzyme Microb Technol, 27(9), 691-697.
- . Biotechnol Prog, 16(6), 1098-1105.
- . ANALYST, 124(2), 157-162.
- . CRITICAL REVIEWS IN BIOTECHNOLOGY, 19(4), 277-316.
- . SynBio, 2(2), 190-205.
- . Applied Microbiology and Biotechnology.
- . Applied Microbiology and Biotechnology.
- . Faraday Discuss., 183, 463-487.
- . Biology, 7(1).
Chapters
- Microbial Consortia: Concept and Application in Fruit Crop Management In Srivastava AK & Hu C (Ed.), Fruit Crops Diagnosis and Management of Nutrient Constraints (pp. 353-366). Elsevier
- , Fruit Crops (pp. 353-366). Elsevier
- Understanding the behaviour of pathogenic cells: proteome and metabolome analyses. In Griffiths M (Ed.), Understanding Pathogen Behaviour (pp. 3-52). CRC Press
- Proteome and metabolome analyses for food authentication In Lees M (Ed.), Food authenticity and traceability [electronic resource] (pp. 71-100). Woodhead Publishing
- , Metabolic Profiling: Its Role in Biomarker Discovery and Gene Function Analysis (pp. 9-38). Springer US
- Metabolome and proteome profiling for microbial characterization: Mass spectrometric applications. In Harrigan GG & Goodacre R (Ed.), Metabolic Profiling (pp. 9-38). Springer
- , Food Authenticity and Traceability (pp. xiii-xvii). Elsevier
- , Food Authenticity and Traceability (pp. 71-100).
Conference proceedings papers
- . SURFACE AND INTERFACE ANALYSIS, Vol. 45(1) (pp 290-293)
- . SURFACE AND INTERFACE ANALYSIS, Vol. 45(1) (pp 255-259)
- ANYL 380-Matrix effects in biomolecular imaging using ToF-SIMS. ABSTR PAP AM CHEM S, Vol. 238
- BIOT 402-Seeking the message in the medium: Metabolic footprints of microalgal cultivations for bioenergy. ABSTR PAP AM CHEM S, Vol. 238
- . APPLIED SURFACE SCIENCE, Vol. 255(4) (pp 1264-1270)
- . APPLIED SURFACE SCIENCE, Vol. 255(4) (pp 922-925)
- . APPL SURF SCI, Vol. 255(4) (pp 1599-1602)
- Proteome and metabolome analyses for rapid authentication of food.. Rapid Methods Europe 2005 (pp 29-48). Noordwijk aan Zee, the Netherlands, 24 May 2005 - 25 May 2005.
- Electrospray-ionization mass spectrometry of protein mixtures: Influence of instrumental settings and proteomic implications.. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol. 229 (pp U185-U185)
- Metabolomic investigations using laser desorption ionisation mass spectrometry on porous silicon. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol. 229 (pp U245-U245)
- Metabolite and protein profiling of whole cells using soft-ionization mass spectrometry for rapid microbial characterization.. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol. 225 (pp U199-U199)
- Rapid, high-throughput microbial characterization by metabolite and protein profiling of whole cells using soft-ionization mass spectrometry.. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol. 224 (pp U199-U199)
- Measurement of mycelial biomass in a submerged bioprocess using near infrared spectroscopy.. NIRS-鈥99 - Proceedings of the 9th International conference on Near-infrared spectroscopy (pp 429-433). Chichester, UK
Preprints
- Research group
-
Our current research focus is in developing and establishing microalgae-based value chains and processes that can be carbon negative and offer environmentally sustainable solutions with wider impact in the chemicals, materials, energy, food and health sectors. We are working with a diverse group of microalgae, including Chalmydomonas sp., Chlorella sp., Dunaliella salina, Scenedesmus sp., Phaeodactylum tricornutum, Nannochloropsis oceanica, Porphyridium purpureum, etc. The investigations include molecular level understanding and identification of relevant metabolic pathways, metabolic modelling, systems level characterisations, chemical and biochemical process engineering, techno-economic assessments, resource utilisation towards a circular bio-economy. We have been funded by UKRI BBSRC and EPSRC o develop an understanding of lipid accumulation in microalgae towards sustainable biofuels production, and by EU H2020, through an MSCFA to study nutrient uptake by microalgae.
We have identified key delineating features suitable for lipid accumulation in marine microalgae that would help in selecting appropriate strains and process conditions suitable for oil production in microalgae []. We have studied nutrient uptake in the context of lipid accumulation in P. tricornutum and N. oceanica [], as well as in N. salina and D. salina [ and have reported on the differences in the capacity between species to respond to the supply of nitrogen and exposure to high intensity light towards increased lipid accumulation. We have reported on cryopreservation of Chlorella to preserve its lipid accumulating capacity []. We are working towards a better understanding of carbon uptake by microalgae [ to enable appropriate interventions for carbon capture and utilisation. Methods to characterise dissolved inorganic carbon [], intracellular biochemical compositions [,], proteomic and metabolomic data acquisition in microalgae [,,,] have been established. We are developing strategies for microalgae based microbial co-culture systems for better bioproduct formations [, ]. Accumulation of the health-promoting polyunsaturated fatty acids, such as EPA, in the diatom, P. tricornutum have been characterised in pilot-scale outdoor PBRs, as a part of BBSRC funded research (PHYCBIV-28), as well as cell lysis for extraction of products from microalgae (PHYCBIV-13). The development of a biorefinery concept with microalgal cultivations to sustainably sequester CO 2 and produce economically viable processes is being explored with the diatom P. tricornutum [, ]. These include downstream processing considerations [, ].
We wish to build on these findings and develop relevant microalgae-based value chains and materials to enable sustainable interventions in diverse sectors, including renewable energy generation [, ], agriculture [], aquaculture practice, food production, nutrition, health, bulk and fine chemical manufacture. These interventions are aimed at developing environmentally inclusive, economically impacting routes to future food security, chemical manufacturing, health and energy generation. We have wider expertise in microbial characterisations, omic analyses, mass spectrometric imaging, bioinformatics, bioprocess monitoring and microbial process engineering.
- Grants
- Teaching activities
-
- CPE403/6030 - Environment: Gaseous Emissions
- CPE401 - Research Project (MEng)
- CPE6009 鈥 Bio-energy
- CPE6016 鈥 Bioanalytical Techniques (MSc BBE)