Dr Alice Pyne
MSci, MRes, EngD
School of Chemical, Materials and Biological Engineering
Senior Lecturer in Soft Matter & Polymers
+44 114 222 5969
Full contact details
School of Chemical, Materials and Biological Engineering
Sir Robert Hadfield Building
Mappin Street
91直播
S1 3JD
- Profile
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Alice is a Senior Lecturer in Polymers & Soft Matter and head of the Henry Royce Nanocharacterisation laboratory. Her research focusses on developing high-resolution Atomic Force Microscopy (AFM) imaging methods for structural characterisation of biomolecules in solution. She has pioneered single-molecule imaging studies of the DNA double helix, including unique time-resolved imaging at the atomic scale, showing DNA molecules twisting and 鈥渄ancing鈥 in ways that had not previously been imaged. Alice has a keen interest in bioimage analysis, and has developed an automated image analysis pipeline, , that combines AFM image correction, molecule identification, and tracing into a single tool.
Prior to moving to 91直播, Alice completed an EngD in Biophysics at the London Centre for Nanotechnology, UCL and was awarded two postdoctoral fellowships during which she established a track record in high resolution biomolecular AFM.
- Qualifications
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- EngD Biophysics, University College London & the National Physical Laboratory, High Resolution Atomic Force Microscopy of Functional Biological Molecules (2015)
- MRes (Hons) Distinction, UCL, Molecular Modelling and Materials Science (2011)
- MSci (Hons) Physics, University of Bristol (2009)
- Research interests
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Alice鈥檚 current projects combine high-resolution AFM and cutting-edge bioimage analysis to determine how structural and conformational heterogeneity in individual DNA molecules affects fundamental biological processes.
- Publications
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Journal articles
- . Biophysical Journal, 123(3), 320a-320a.
- . Biophysical Journal, 122(3), 9a-10a.
- . Methods Mol Biol, 2476, 43-62.
- . APL Bioengineering, 5.
- . Nature Communications, 12.
- . Methods.
- , 225-235.
- . ACS Sensors, 5(10), 3133-3139.
- . The Analyst, 144(23), 6944-6952.
- . Nature Communications, 10(1), ---.
- . Nanoscale, 11(42), 20072-20080.
- . The EMBO Journal, 38(4).
- . Scientific Reports, 8(1).
- . Scientific Reports, 8(1).
- . ACS Synthetic Biology, 7(3), 767-773.
- . Molecular Immunology, 89, 154-154.
- . Chemical Science, 8(2), 1105-1115.
- . Angewandte Chemie, 128(37), 11272-11275.
- . Angewandte Chemie International Edition, 55(37), 11106-11109.
- . Small, 10(16), 3257-3261.
- . Proceedings of the National Academy of Sciences, 110(22), 8918-8923.
- . Nano Letters, 12(7), 3846-3850.
- . Archives of Histology and Cytology, 72(4/5), 209-215.
- . The EMBO Journal.
- . Physical Chemistry Chemical Physics.
- . Small.
- . Nature Communications, 14(1).
- . Chemical Communications, 52(84), 12454-12457.
Chapters
- , Nucleic Acids in Chemistry and Biology (pp. E1-E50). The Royal Society of Chemistry
- , Methods in Molecular Biology (pp. 47-60). Springer New York
- In Blackburn GM, Gait MJ, Loakes D & Williams DM (Ed.) The Royal Society of Chemistry
Conference proceedings papers
- Probing antimicrobial mechanisms for effective strategies to overcome resistance. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Vol. 48 (pp S223-S223)
- Visualisation of DNA conformational changes in situ at nanometre resolution. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Vol. 46 (pp S369-S369)
- A combinatorial single-molecule study of ligand-gated ion channels and monoclonal antibodies. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Vol. 46 (pp S371-S371)
- Investigating the mechanism of action of a novel antimicrobial peptide on live E. coli cells. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Vol. 46 (pp S389-S389)
Software / Code
Datasets
Other
Preprints
- Research group
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PhD Students:
- Daniel Rollins (AFM analysis of supercoiled DNA interactions)
- Mingxue Du (TopoStats: Automating AFM analysis of DNA & protein interactions)
- Libby Holmes (Complex DNA structures in cancer)
- Max Gamil (Deep probabilistic models for analysing complex DNA structures)
PhD Students (UCL):
Kavit Main (Single molecule insights into DNA-Topoiomerase interactions)
- Teaching activities
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- BIE103 (Introduction to Bioengineering)
- MAT360 (Research skills)
- MAT408 (Bioimaging)
- MEC438 (Preparation for Practice)
- Professional activities and memberships
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Professional Activities:
- British Biophysical Society committee member
- Royal Microscopical Society Data Analysis in Imaging committee member
- Wiley editorial board member
- Co-director of the INSIGNEO biomedical imaging pillar
- Head of the Henry Royce Nanocharacterisation Laboratory
- Co-organiser of the international Royal Microscopical Society conference series
- Co-organiser of the first international Royal Microscopical Society 鈥樷 conference
Awards:
- Young Investigator Award - DNA, UK Nucleic Acids Forum 2019
- Journal of Molecular Recognition Award 2014
- National Physical Laboratory鈥檚 Raleigh Prize 2013
Links