Professor Andrew Peden
School of Biosciences
Professor
+44 114 222 2312
Full contact details
School of Biosciences
D06, Florey Building
Florey Building
Western Bank
91Ö±²¥
S10 2TN
- Profile
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- 2023-present: Professor, School of Bioscience, the University of 91Ö±²¥.
- 2012-2018: Lecturer, Department of Biomedical Science, the University of 91Ö±²¥.
- 2019-present: Senior Lecturer, School of Biosciences, the University of 91Ö±²¥.
- 2012-2018: Lecturer, Department of Biomedical Science, the University of 91Ö±²¥
- 2006-2012: Principal Investigator, University of Cambridge (MRC CDA).
- 2001-2005: Postdoctoral fellow, Genentech Inc.
- 2000-2001: Postdoctoral fellow, Stanford University (Wellcome Trust Research Fellowship).
- 1995-1999: PhD, University of Cambridge.
- 1991-1995: BSc., University of Edinburgh.
- Research interests
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Constitutive secretion is a conserved process required for the delivery of newly synthesised proteins and lipids to plasma membrane as well as the exocytocis of extracellular factors such as cytokines, lipoproteins and antibodies.
My lab is interested in identifying and characterising the pathways and machinery involved in constitutive secretion.
Elucidating the post-Golgi pathways and machinery required for constitutive secretion
Constitutive secretion is required for many biologically important processes, such as inflammation (cytokine secretion), adaptive immunity (IgG secretion), tissue remodelling (extracellular matrix secretion) and cholesterol homeostasis (lipoprotein particle secretion). Perturbations in the secretion of these factors can cause disease.
For example dysregulation of antibody secretion plays a significant role in the development of diseases such Amyloidosis, Macroglobulinemia and Monoclonal Gammopathies. Thus having the ability to modulate the secretion of various cellular factors has significant therapeutic potential.
My lab has 3 main aims:
1) To elucidate the role of SNAREs in constitutive secretion
SNAREs are a familyof proteins required for the fusion of membranes and there are 38 encoded in the human genome. Each SNARE is localised to a specific compartment within the cell and required a defined set of fusion steps.
However, it is not known which SNAREs are required for the fusion of secretory vesicles with the plasma membrane. To address this we have developed novel assays for measuring constitutive secretion and have used them in conjunction with targeted siRNA screens in both mammalian and Drosophila based systems.
We have shown that in mammals the post-Golgi SNAREs SNAP29 and STX19 and are required for constitutive secretion. SNAP29 is mutated in the human disorder CEDNIK so our work may help shed light on the molecular details of this disease.
Many questions remain regarding the role of SNAREs in constitutive secretion. For example, how are the functions of SNAP29 and STX19 coordinated, how is constitutive secretion regulated, how are SNAREs packaged into post-Golgi secretory carriers. We are addressing these questions using live cell imaging, secretion assays, proteomics and structural based approaches.
Movie showing RPE-1 cells secreting the GFP tagged protein we use to measure secretion. The tubular structures emanating from the centre of the cells are secretory carriers which will eventually fuse with the plasma.
2) To identify and characterise novel machinery required for post-Golgi trafficking and antibody secretion
Very little is known about the machinery required for budding, transporting, docking and fusing post-Golgi transport vesicles. To identify this machinery we are using two approaches.
Firstly we are using a screening approach (RNAi or chemical) where we will make use of our novel secretion assays in mammalian and Drosophila cell lines. In the second approach we plan to use proteomics to quantify changes in protein expression associated with plasma cell differentiation.
3) To understand the molecular interactions required for post-Golgi trafficking and secretion
To elucidate the molecular interactions involved in post-Golgi trafficking and secretion we are tacking a structural approach in collaboration with Professor David Owen.
We are particularly interested in understanding how post-Golgi SNAREs are packaged into transport vesicles.
We have recently shown that the clathrin coated vesicle protein CALM directly binds to the coiled-coil domains of the R-SNAREs VAMPs 2/3/8 and facilitates their internalisation.
- Publications
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Show: Featured publications All publications
Featured publications
Journal articles
- . eLife.
- . Proceedings of the National Academy of Sciences, 120(2).
- . Communications Biology, 5.
- . Journal of Cell Science, 134(10).
- , 115-129.
- . Science, 370(6521), ---.
- . Journal of Cell Science, 131(20).
- . Nature Communications, 9(1).
- . Frontiers in Pharmacology, 8, 796-796.
- . PLoS Genetics, 13(4).
- . The FASEB Journal, 30(12), 4083-4097.
- . Cell Reports, 14(11), 2624-2636.
- . Cellular Microbiology, 18(1), 80-96.
- . Journal of Neuroscience, 35(19), 7643-7653.
- . PLoS ONE, 9(10).
- . Molecular Biology of the Cell, 24(15), 2378-2388.
- . Journal of Cell Science, 126(20), 4647-4658.
- . Developmental Cell, 22(5), 979-988.
- . Cell, 147(5), 1118-1131.
- . Traffic, 11(9), 1191-1204.
- . EMBO Journal, 29(2), 304-314.
- . EMBO Reports, 10(8), 851-856.
Preprints
All publications
Journal articles
- . eLife.
- . EMBO reports.
- . iScience, 107056-107056.
- . Proceedings of the National Academy of Sciences, 120(2).
- . Life Science Alliance, 6(1), e202201449-e202201449.
- . Communications Biology, 5.
- . Proc Natl Acad Sci U S A, 119(18), e2201646119.
- . Journal of Biological Chemistry, 297(6).
- . Nat Commun, 12(1), 5828.
- . Frontiers in Cell and Developmental Biology, 9.
- . Journal of Cell Science, 134(10).
- . J Cell Sci.
- , 115-129.
- . Science, 370(6521), ---.
- . Journal of Biotechnology, 312, 11-22.
- . Open Forum Infectious Diseases, 6(Supplement_2), S904-S904.
- . Journal of Biological Chemistry, 294(42), 15418-15434.
- . Toxicon, 156, S90-S90.
- . Toxicon, 156, S25-S25.
- . Journal of Cell Science, 131(20).
- . Nature Communications, 9(1).
- . Frontiers in Pharmacology, 8, 796-796.
- . PLoS Genetics, 13(4).
- . The FASEB Journal, 30(12), 4083-4097.
- . Cell Reports, 14(11), 2624-2636.
- . Cellular Microbiology, 18(1), 80-96.
- . Journal of Neuroscience, 35(19), 7643-7653.
- . PLoS ONE, 9(10).
- . Molecular Biology of the Cell, 24(15), 2378-2388.
- . PLoS ONE, 8(5).
- . Checkpoint Pathways Regulating Immune Responses.
- . Journal of Cell Science, 126(20), 4647-4658.
- . Current Biology, 22(18), 1711-1716.
- . Developmental Cell, 22(5), 979-988.
- . Cell, 147(5), 1118-1131.
- . Journal of Cell Science, 124(3), 469-482.
- . Molecular Biology of the Cell, 22(1), 54-65.
- . The Journal of Experimental Medicine, 207(10), i29-i29.
- . Journal of Cell Biology, 190(6), 1023-1037.
- . Molecular Biology of the Cell, 21(17), 3041-3053.
- . Traffic, 11(9), 1191-1204.
- . Cell, 141(2), 222-224.
- . EMBO Journal, 29(2), 304-314.
- . Human Molecular Genetics, 19(6), 1009-1018.
- . International Journal of Biochemistry and Cell Biology, 41(2), 330-340.
- . Journal of Biological Chemistry, 284(3), 1790-1802.
- . EMBO Reports, 10(8), 851-856.
- . Proceedings of the National Academy of Sciences of the United States of America, 105(49), 19561.
- . Proceedings of the National Academy of Sciences of the United States of America, 105(43), 16513-16518.
- . Development, 135(8), 1415-1425.
- . Proceedings of the National Academy of Sciences of the United States of America, 103(36), 13560.
- . Molecular Biology of the Cell, 17(9), 4014-4026.
- . Proceedings of the National Academy of Sciences of the United States of America, 103(27), 10283-10288.
- . Molecular Biology of the Cell, 16(11), 5356-5372.
- . Journal of Molecular Biology, 348(5), 1153-1162.
- . Molecular Biology of the Cell, 16(2), 849-860.
- . Methods in Enzymology, 403, 512-525.
- . Molecular Biology of the Cell, 15(12), 5369-5382.
- . Journal of Biological Chemistry, 279(32), 33430-33437.
- . Molecular Biology of the Cell, 15(8), 3530-3541.
- . J Biol Chem, 279(24), 25430-25439.
- . Molecular Biology of the Cell, 15(5), 2218-2229.
- . Journal of Cell Biology, 164(7), 1065-1076.
- . Mammalian Genome, 14(3), 157-167.
- . Journal of Cell Biology, 156(2), 327-336.
- . Journal of Biological Chemistry, 276(52), 49183-49187.
- . Nature, 409(6822), 839-841.
- . Blood, 94(1), 146-155.
- . Human Molecular Genetics, 8(2), 323-330.
- . Neuron, 21(1), 111-122.
- . Journal of Cell Biology, 137(4), 835-845.
- . ALTEX.
- . Traffic.
Chapters
Conference proceedings papers
- Sec22b is indispensable for plasma cell maintenance and antibody secretion. EUROPEAN JOURNAL OF IMMUNOLOGY, Vol. 51 (pp 9-9)
- Mechanisms Controlling the Motility and Fusion of Rab6-positive Exocytotic Carriers. MOLECULAR BIOLOGY OF THE CELL, Vol. 22
- The endosome equilibrium: Pathways to and from early endosomes. MOLECULAR BIOLOGY OF THE CELL, Vol. 13 (pp 284A-284A)
Preprints
- , eLife Sciences Publications, Ltd.
- , eLife Sciences Publications, Ltd.
- , eLife Sciences Publications, Ltd.
- , Cold Spring Harbor Laboratory.
- , Cold Spring Harbor Laboratory.
- , Cold Spring Harbor Laboratory.
- , Cold Spring Harbor Laboratory.
- , Cold Spring Harbor Laboratory.
- , Cold Spring Harbor Laboratory.
- , Cold Spring Harbor Laboratory.
- , Cold Spring Harbor Laboratory.
- Research group
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Collaborators
- David James (University of 91Ö±²¥)
- Mark Collins (University of 91Ö±²¥)
- Bazbek Davletov (University of 91Ö±²¥)
- Luke Chamberlain (University of Strathclyde)
- Clare Futter (University College London)
PhD studentship opportunities
We advertise PhD opportunities (Funded or Self-Funded) on FindAPhD.com
- Grants
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- BBSRC
- MRC
- Medimmune
- UCB
- Teaching activities
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- BIS213: Skills in Biomedical Science (Herceptin practical co-ordinator).
- BIS225: Molecular and Cell Biology.
- BMS376: Membrane Dynamics in Health and Disease.
- Supervise level 3 research projects.
- Supervise level 4 and masters research projects.
- Professional activities and memberships
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- 2023-present BBSRC Committee D Core Member
- 2021-present Member of the WHO COVID-19 assays group
- 2018-present Carnegie Trust research assessor
Links